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Our lab is interested in the regulation of cell division. Cell division is a fundamental process by which all living things propagate. As mammalian cells progress into mitosis (M-phase) and cytokinesis (C-phase), the cell undergoes dramatic reorganization in its structure and biochemical state in a short period of time, and the dynamics of this process are poorly understood.  By the entry into mitosis, almost all aspects of the cell’s interior are changed under the influence of the master mitotic kinase Cdk1. Similarly; cell surface morphology undergoes dramatic reshaping at the onset of mitosis. As adherent cells enter mitosis they transiently lose their adherence and round up. At cytokinesis the daughter cells spread back to regain their interphase morphology.

 

 

 

 

The major questions that we aim to understand are how cell cycle dependent changes are regulated and how the changes on the cell membrane are coordinated with cell’s interior to drive cell division and how the cell is communicating with its extracellular environment during division. Towards this goal, we apply quantitative proteomic techniques to examine the biochemical profile of different cellular compartments as mammalian cells progress through mitosis and cytokinesis, during normal physiology, and in response to perturbation of different cellular components by drugs. In parallel we perform microscopy-based assays and cell biology techniques to determine the underlying mechanisms of the biochemical behaviors observed in the proteomic analyses.

 

Proteomic Analysis of Cell Division, EMBO YIP Talk - Nurhan Özlü